Restriction Enzymes
New England Biolabs, Inc. SfiI – 15000 units
A restriction endonuclease that recognizes the sequence GGCCN_NNN^NGGCC.
New England Biolabs, Inc. MluI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence A^CGCGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. SmaI – 10000 units
A restriction endonuclease that recognizes the sequence CCC_^GGG.
New England Biolabs, Inc. PstI – 10000 units
A restriction endonuclease that recognizes the sequence C_TGCA^G.
New England Biolabs, Inc. SmaI – 2000 units
A restriction endonuclease that recognizes the sequence CCC_^GGG.
New England Biolabs, Inc. BstBI – 2500 units
A restriction endonuclease that recognizes the sequence TT^CG_AA.
New England Biolabs, Inc. BfaI – 500 units
A restriction endonuclease that recognizes the sequence C^TA_G.
New England Biolabs, Inc. BsiWI-HF – 1500 units
A high fidelity restriction endonuclease that recognizes the sequence C^GTACG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BbsI-HF – 1500 units
A high fidelity restriction endonuclease that recognizes the sequence GAAGAC(N)2^. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. AleI-v2 – 2500 units
AleI-v2 is an engineered and improved version of AleI that recognizes the sequence CACNN^NNGTG.
New England Biolabs, Inc. TspMI – 200 units
A restriction endonuclease that recognizes the sequence C^CCGG_G.
New England Biolabs, Inc. BclI-HF – 3000 units
A high fidelity restriction endonuclease that recognizes the sequence T^GATCA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. Exonuclease V (RecBCD) – 5000 units
Exonuclease V, a RecBCD complex from E. coli has several different enzyme activities, including an ATP-dependent single-stranded DNA endonuclease activity, ss- and ds- DNA exonuclease activity. The hydrolysis in each case is bi-directional (from both the 3' and 5' ends) and processive, producing oligonucleotides. All Exonuclease V activities have divalent cation requirements. Mg2+ is required for the exonuclease activity, while Ca2+ inhibits the exonuclease activity and allows double-stranded DNA unwinding (helicase activity) without hydrolysis.
New England Biolabs, Inc. RNase Inhibitor, Human Placenta – 2000 units
RNase Inhibitor, Human Placenta is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor, Human Placenta is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014.
New England Biolabs, Inc. Endonuclease V – 250 units
Endonuclease V is a repair enzyme found in E. coli that recognizes deoxyinosine, a deamination product of deoxyadenosine in DNA. Endonuclease V, often called deoxyinosine 3 endonuclease, recognizes DNA containing deoxyinosines (paired or not) on double-stranded DNA, single-stranded DNA with deoxyinosines and to a lesser degree, DNA containing abasic sites (ap) or urea, base mismatches, insertion/deletion mismatches, hairpin or unpaired loops, flaps and pseudo-Y structures. It is believed that Endonuclease V needs another protein to repair the DNA, as it does not remove the deoxyinosine or the damaged bases. Endonuclease V cleaves the second phosphodiester bonds 3' to the mismatch of deoxyinosine, leaving a nick with 3'-hydroxyl and 5'-phosphate.